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| Registros recuperados: 36 | |
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| Goudenege, David; Travers, Marie-agnes; Lemire, Astrid; Petton, Bruno; Haffner, Philippe; Labreuche, Yannick; Tourbiez, Delphine; Mangenot, Sophie; Calteau, Alexandra; Mazel, Didier; Nicolas, Jean-louis; Jacq, Annick; Le Roux, Frederique. |
| Oyster diseases caused by pathogenic vibrios pose a major challenge to the sustainability of oyster farming. In France since 2012 a disease affecting specifically adult oysters has been associated with the presence of Vibrio aestuarianus. Here, by combining genome comparison, phylogenetic analyses and high throughput infections of strains isolated before or during the recent outbreaks, we show that virulent strains cluster into two V. aestuarianus lineages independently of the sampling dates. The bacterial lethal dose was not different between strains isolated before or after 2012. Hence the emergence of a new highly virulent clonal strain is unlikely. Each lineage comprises nearly identical strains, the majority of them being virulent, suggesting that... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00226/33685/32530.pdf |
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| Bruto, Maxime; Labreuche, Yannick; James, Adele; Piel, Damien; Chenivesse, Sabine; Petton, Bruno; Polz, Martin F.; Le Roux, Frederique. |
| Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pathogens is scarce. Here we ask how widespread is virulence among ecologically differentiated Vibrio populations, and what is the nature and frequency of virulence genes within these populations? We use a combination of population genomics and molecular genetics to assay hundreds of Vibrio strains for their virulence in the oyster Crassostrea gigas, a unique animal model that allows high-throughput infection assays. We show that within the diverse Splendidus... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00452/56370/57967.pdf |
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| Thebault, Anne; Martin, Anne-genevieve; Le Coguic, Yvette; Hirata, Toshio; Tige, Gilbert; Pichot, Yves; Grizel, Henri; Berthe, Franck; Cochennec, Nathalie; Renault, Tristan; Le Roux, Frederique. |
| Le réseau REPAMO (Réseau de Pathologie des Mollusques) est un réseau de santé animale, qui s'occupe des coquillages marins du littoral français métropolitain. Les mollusques marins des côtes françaises représentent une ressource par la pêche, mais sont surtout une part importante de l'aquaculture. Les pertes liées aux maladies dans ce domaine d'activité sont aujourd'hui considérées comme l'un des principaux facteurs limitants de son développement au niveau mondial. Deux exemples récents suffisent à illustrer ce danger pour l'ostréiculture française : • La culture de l''huître plate Ostrea edulis, déjà atteinte par un parasite, Marteilia refringens, est passée en quelques années de 20 000 tonnes à 1800 tonnes, suite à une épizootie. En effet à la fin des... |
| Tipo: Text |
Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00098/20903/18522.pdf |
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| Foulon, Valentin; Le Roux, Frederique; Lambert, Christophe; Huvet, Arnaud; Soudant, Philippe; Paul-pont, Ika. |
| Microplastics collected at sea harbour a high diversity of microorganisms including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for potentially pathogenic microorganisms. In the present study we investigated the adhesion dynamics of Vibrio crassostreae on polystyrene microparticles (micro-PS) using electronic and fluorescence microscopy techniques. Micro-PS were incubated with bacteria in different media (Zobell culture medium and artificial seawater) with or without natural marine aggregates. The highest percentage of colonised particles (38-100%) was observed in Zobell culture medium, which may be related to nutrient availability for production of pili and exopolysaccharide adhesion structures.... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00351/46210/45908.pdf |
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| Goudenege, David; Labreuche, Yannick; Krin, Evelyne; Ansquer, Dominique; Mangenot, Sophie; Calteau, Alexandra; Medigue, Claudine; Mazel, Didier; Polz, Martin F.; Le Roux, Frederique. |
| Vibrio nigripulchritudo is an emerging pathogen of farmed shrimp in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have suggested that pathogenicity is linked to particular lineages. Here, we performed high-throughput sequencing-based comparative genome analysis of 16 V. nigripulchritudo strains to explore the genomic diversity and evolutionary history of pathogen-containing lineages and to identify pathogen-specific genetic elements. Our phylogenetic analysis revealed three pathogen-containing V. nigripulchritudo clades, including two clades previously identified from New Caledonia and one novel clade comprising putatively... |
| Tipo: Text |
Palavras-chave: Horizontal gene transfer; Nigritoxin; Phylogeny; Shrimp; Virulence; Vibrio. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00160/27117/28369.pdf |
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| Petton, Bruno; Bruto, Maxime; James, Adele; Labreuche, Yannick; Alunno-bruscia, Marianne; Le Roux, Frederique. |
| Successive disease outbreaks in oyster (Crassostrea gigas) beds in France have resulted in dramatic losses in production, and subsequent decline in the oyster-farming industry. Deaths of juvenile oysters have been associated with the presence of a herpes virus (OsHV-1 µvar) and bacterial populations of the genus Vibrio. Although the pathogenicity of OsHV-1 µvar, as well as several strains of Vibrio has been demonstrated by experimental infections, our understanding of the complexity of infections occurring in the natural environment remains limited. In the present study, we use specific-pathogen-free (SPF) oysters infected in an estuarine environment to study the diversity and dynamics of cultured microbial populations during disease expression. We observe... |
| Tipo: Text |
Palavras-chave: Pacific oysters; Summer mortality; Herpes virus; Vibrio pathogenicity; Experimental infection. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00271/38220/36377.pdf |
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| Nguyen, An Ngoc; Disconzi, Elena; Charriere, Guillaume; Destoumieux-garzon, Delphine; Bouloc, Philippe; Le Roux, Frederique; Jacq, Annick. |
| CsrBs are bacterial highly conserved and multiple-copy noncoding small RNAs (sRNAs) that play major roles in cell physiology and virulence. In the Vibrio genus, they are known to be regulated by the two-component system VarSNarA. They modulate the well-characterized quorum sensing pathway controlling virulence and luminescence in Vibrio cholerae and Vibrio harveyi, respectively. Remarkably, Vibrio tasmaniensis LGP32, an oyster pathogen that belongs to the Splendidus Glade, was found to have four copies of csrB, named csrB1-4, compared to two to three copies in other Vibrio species. Here, we show that the extra csrB4 copy results from a csrB3 gene duplication, a characteristic of the Splendidus Glade. Interestingly, csrB genes are regulated in different... |
| Tipo: Text |
Palavras-chave: Bacterial gene regulation; Bacterial sRNAs; Transcriptomics; Vibrio pathogenic to oysters; Host-pathogen interactions. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00476/58785/61319.pdf |
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| Beliaeff, Benoit; Chim, Liet; Della Patrona, Luc; Goyard, Emmanuel; Herlin, Jose; Labreuche, Yannick; Walling, Emilie; Ansquer, Dominique; Brun, Pierre; Castex, Mathieu; Coatanea, Denis; Courties, Claude; De Lorgeril, Julien; Dufour, Robert; Frappier, Julie; Goarant, Cyrille; Huber, Matthias; Lemaire, Pierrette; Lemonnier, Hugues; Loubersac, Lionel; Le Roux, Frederique; Lucas, Ronan; Patrois, Jacques; Peignon, Jean-marie; Pham, Dominique; Ramage, Yannick; Soulard, Benoit; Vic, Marlene; Vourey, Elodie; Wabete, Nelly. |
| New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government... |
| Tipo: Text |
Palavras-chave: Shrimp farming; Technical and scientific support; Vibriosis; Water quality; Pathology; Ecophysiology; Genetics; Zoosanitary monitoring and procedures; Multifactorial and multidisciplinary approach. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00143/25438/23591.pdf |
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| Le Roux, Frederique. |
| Global changes linked to increases in temperature and ocean acidification, but also to more direct anthropogenic influences such as aquaculture, have caused a worldwide increase in the reports of Vibrio-associated illnesses affecting humans and also animals such as shrimp and molluscs. Investigation of the emergence of Vibrio pathogenesis events requires the analysis of microbial evolution at the gene, genome and population levels, in order to identify genomic modifications linked to increased virulence, resistance and/or prevalence, or to recent host shift. From a more applied point of view, the elucidation of virulence mechanisms is a prerequisite to devising prophylactic methods to fight infectious agents. In comparison with human pathogens, fairly... |
| Tipo: Text |
Palavras-chave: Écologie; Émergence; Évolution; Invertébrés marins; Vibriose; Virulence; Ecology; Emergence; Evolution; Marine invertebrate; Vibriosis; Virulence. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00344/45560/46722.pdf |
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| Parizadeh, Leila; Tourbiez, Delphine; Garcia, Celine; Haffner, Philippe; Degremont, Lionel; Le Roux, Frederique; Travers, Marie-agnes. |
| Although vibrios are frequently associated with marine organisms mortality outbreaks, knowledge on their ecology and pathogenicity is sparse, thus limiting disease management and prophylactic strategies. Here, we investigated V. aestuarianus infection onset and progression in the wild, taking advantage of a “claire” pond: a semi‐closed system with limited seawater renewal, theoretically more adapted to disease transmission. We showed a positive association of the bacteria with oysters, which can constitute a reservoir for the bacteria in the winter. Moreover, passage through oysters was found to be necessary for experimental disease reproduction as vibrios shedding from diseased oysters has higher infectivity than from in vitro grown. We next developed an... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00446/55761/57423.pdf |
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| Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno. |
| Nous disposons aujourd'hui d'o utils diagnostiques sensibles et spécifiques (PCR, HIS ... ) et d'un protocole de transmission de l' infection qui permettent d'assurer un suivi des productions larvaires en écloserie de bivalves pour les espèces considérées comme sensibles aux infections à herpèsvirus, mais également pour de nouvelles espèces. En effet, des mortalités massives (100 %) ont été rapportées dans une écloserie de coquille St-Jacques en Bretagne et ont pu être associées à la détection de OsHV -1. Les résultats obtenus concernant cette espèce, Pecten maximus, complètent et reflètent l'ensemble des travaux réalisés. |
| Tipo: Text |
Palavras-chave: Pathologie; Epidémiologie; Virus; Herpès virus; Bivalves; Crassostrea gigas; Ruditapes philippinarum; Pecten maximus. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00043/15472/12851.pdf |
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| Renault, Tristan; Le Roux, Frederique; Berthe, Franck; Robert, Maeva; Chollet, Bruno; Gerard, Andre. |
| Le programme 4 portant sur la pathologie des coquillages dans le cadre d'une gestion durable des productions ostréicoles, comporte 2 actions principales réparties en une étude d'épidémiologie analytique et descriptive des agents pathogènes (Action 1), et l'étude des relations hôtes/agents pathogènes (Action 2). Les résultats 2001 présentés ici sont relatifs à ces 2 actions, avec notamment des informations sur les infections à virus de type herpès, et de recherche de bactéries pathogènes chez Crassostrea gigas lors des épisodes de mortalités estivales. Par ailleurs, l'émergence d'un programme national, intitulé MOREST (pour Mortalités Estivales) sur l' huître creuse C. gigas va permettre de rappeler le contexte scientifique des relations hôtes/pathogènes. |
| Tipo: Text |
Palavras-chave: Pathologie; Agents pathogènes; Epidémiologie; Huître creuse; Crassostrea gigas. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00199/31011/29414.pdf |
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| Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine. |
| Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the... |
| Tipo: Text |
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf |
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| Le Roux, Frederique; Zouine, Mohamed; Chakroun, Nesrine; Binesse, Johan; Saulnier, Denis; Bouchier, Christiane; Zidane, Nora; Ma, Laurence; Rusniok, Christophe; Lajus, Aurelie; Buchrieser, Carmen; Medigue, Claudine; Polz, Martin F.; Mazel, Didier. |
| P>Vibrio splendidus is a dominant Vibrio species in seawater presenting a remarkable genetic diversity; several strains have been linked to invertebrate's mortality. We report the complete genome sequence of V. splendidus LGP32, an oyster pathogen, and its comparison with partial genome sequences from related strains. As is typical for the genus, V. splendidus LGP32 contains two chromosomes (3.29 and 1.67 Mb) and most essential cellular processes are encoded by chromosome 1. Comparison with two other V. splendidus partial genome sequences (strains 12B01 and Med222) confirms the previously suggested high genotypic diversity within this species and led to the identification of numerous strain-specific regions that could frequently not be assigned to a... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6632.pdf |
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| De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume. |
| Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf |
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| Wegner, K. Mathias; Piel, Damien; Bruto, Maxime; John, Uwe; Mao, Zhijuan; Alunno-bruscia, Marianne; Petton, Bruno; Le Roux, Frederique. |
| Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between different host populations and their locally encountered Vibrio communities. Here, we explore the molecular targets of such co-evolutionary interactions by analyzing the genomes of nine Vibrio strains from the Splendidus-clade showing opposite virulence patterns towards two populations of Pacific oysters introduced into European Wadden Sea. By contrasting Vibrio phylogeny to their host specific virulence patterns, we could identify two core... |
| Tipo: Text |
Palavras-chave: Virulence; Host-pathogen interaction; Local adaptation; Biological invasion; Emerging disease; Wadden Sea. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00512/62409/66669.pdf |
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| Travers, Marie-agnes; Degremont, Lionel; De Lorgeril, Julien; Azema, Patrick; Montagnani, Caroline; Benabdelmouna, Abdellah; Nicolas, Jean-louis; Le Roux, Frederique. |
| En 2012, le nombre de cas de mortalités d’huîtres creuses adultes rapportés dans le cadre du réseau Repamo, et dans lesquels la bactérie Vibrio aestuarianus a été isolée, a fortement augmenté. Afin d’apporter de premiers éléments de réponse concernant cette augmentation, les objectifs de cette étude étaient de déterminer si l’émergence/la ré-émergence de V. aestuarianus était associée (i) à l’apparition d’un génotype bactérien particulier et/ou (ii) à une sensibilité accrue de certains animaux. L’Ifremer dispose d’une collection de souches bactériennes appartenant majoritairement au genre Vibrio et qui comporte différents isolats de V. aestuarianus, collectés depuis 2001 pendant ou hors épisodes de mortalité. La comparaison de ces isolats en termes de... |
| Tipo: Text |
Palavras-chave: Vibrio aestuarianus; Huître creuse; Crassostrea gigas; Bactérie; Ré-émergenece; Virulence; Génome; Statut immunitaire; Génétique. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00229/34037/32430.pdf |
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| Labreuche, Yannick; Chenivesse, Sabine; Jeudy, Alexandra; Le Panse, Sophie; Boulo, Viviane; Ansquer, Dominique; Pages, Sylvie; Givaudan, Alain; Czjzek, Mirjam; Le Roux, Frederique. |
| The Tetraconata (Pancrustacea) concept proposes that insects are more closely related to aquatic crustaceans than to terrestrial centipedes or millipedes. The question therefore arises whether insects have kept crustacean-specific genetic traits that could be targeted by specific toxins. Here we show that a toxin (nigritoxin), originally identified in a bacterial pathogen of shrimp, is lethal for organisms within the Tetraconata and non-toxic to other animals. X-ray crystallography reveals that nigritoxin possesses a new protein fold of the α/β type. The nigritoxin N-terminal domain is essential for cellular translocation and likely encodes specificity for Tetraconata. Once internalized by eukaryotic cells, nigritoxin induces apoptotic cell death through... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00408/51934/52563.pdf |
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| Vanhove, Audrey; Duperthuy, Marylise; Charriere, Guillaume; Le Roux, Frederique; Goudenege, David; Gourbal, Benjamin; Kieffer-jaquinod, Sylvie; Coute, Yohann; Wai, Sun Nyunt; Destoumieux-garzon, Delphine. |
| Vibrio tasmaniensis LGP32, a facultative intracellular pathogen of oyster haemocytes, was shown here to release outer membrane vesicles (OMVs) both in the extracellular milieu and inside haemocytes. Intracellular release of OMVs occurred inside phagosomes of intact haemocytes having phagocytosed few vibrios as well as in damaged haemocytes containing large vacuoles heavily loaded with LGP32. The OMV proteome of LGP32 was shown to be rich in hydrolases (25%) including potential virulence factors such as proteases, lipases, phospholipases, haemolysins and nucleases. One major caseinase/gelatinase named Vsp for vesicular serine protease was found to be specifically secreted through OMVs in which it is enclosed. Vsp was shown to participate in the virulence... |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00199/31009/29459.pdf |
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| Le Roux, Frederique; Wegner, K. Mathias; Polz, Martin F.. |
| Disease dynamics in the wild are influenced by a number of ecological and evolutionary factors not addressed by traditional laboratory-based characterization of pathogens. Here we propose the oyster, Crassostrea gigas, as a model for studying the interaction of the environment, bacterial pathogens, and the host in disease dynamics. We show that an important first step is to ask whether the functional unit of pathogenesis is a bacterial clone, a population, or a consortium in order to assess triggers of disease outbreaks and devise appropriate monitoring tools. Moreover, the development of specific-pathogen-free (SPF) oysters has enabled assessment of the infection process under natural conditions. Finally, recent results show the importance of microbial... |
| Tipo: Text |
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| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00323/43466/46569.pdf |
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| Registros recuperados: 36 | |
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